Title of article
Th1/Th2 balance in canine peripheral blood lymphocytes—A flow cytometric study
Author/Authors
Horiuchi، نويسنده , , Yutaka and Nakajima، نويسنده , , Yuko and Nariai، نويسنده , , Youko and Asanuma، نويسنده , , Hideki and Kuwabara، نويسنده , , Masato and Yukawa، نويسنده , , Masayoshi، نويسنده ,
Pages
7
From page
179
To page
185
Abstract
The canine immune system undergoes continuous remodeling with advancing age. We measured the Th1/Th2 balance in peripheral blood lymphocytes (PBLs) obtained from 23 Beagles ranging in age from 0.5 to 11.8 years by flow cytometric analysis using intracellular cytokine staining. The percentage of CD4 cells producing interferon-gamma (Th1) increased with age. The percentage of CD4 cells producing interleukin-4 (Th2) increased but much less so. In conclusion, we demonstrated that the Th1/Th2 balance in canine peripheral blood could be measured by this flow cytometry technique and the Th1/Th2 balance inclined to dominance of the Th1 subpopulation in PBLs as the dog matured.
Keywords
aging , dog , flow cytometry , Th1/Th2 balance
Journal title
Astroparticle Physics
Record number
2056641
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