Title of article
Construction of an expression vector for improved secretion of canine IL-18
Author/Authors
Sugiura، نويسنده , , Kikuya and Akazawa، نويسنده , , Takashi and Fujimoto، نويسنده , , Mariko and Wijewardana، نويسنده , , Viskam and Mito، نويسنده , , Kai and Hatoya، نويسنده , , Shingo and Taketani، نويسنده , , Shigeru and Komori، نويسنده , , Masayuki and Inoue، نويسنده , , Norimitsu and Inaba، نويسنده , , Toshio، نويسنده ,
Pages
4
From page
388
To page
391
Abstract
To construct a vector for caspase-1 independent expression of canine IL-18, the signal sequence of canine IL-12p40 was fused to the sequence of mature IL-18 on the NdeI restriction site which is located at the 3′ end of the signal sequence. The resulting vector expressed coding protein from transfected mammalian cells. The expressed protein was shown to have IL-18 bioactivity in a INF-γ-inducing assay. These results suggest that the expression vector is the desired tool for advancement of dendritic cell (DC)-based cancer therapy, provided that the vector can successfully be transfected into dendritic cells. We propose a simple and widely applicable method for providing the signal sequence.
Keywords
IL-18 , signal sequence , IL-12p40 , protein expression , INF-?-induction
Journal title
Astroparticle Physics
Record number
2057413
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