Author/Authors :
Nakata، نويسنده , , Junya and Nakahari، نويسنده , , Aiko and Kato، نويسنده , , Yohei and Yamachi، نويسنده , , Nanako and Sato، نويسنده , , Nami and Yonezawa، نويسنده , , Tomohiro and Takahashi، نويسنده , , Chika and Chikazawa، نويسنده , , Seishiro and Hori، نويسنده , , Yasutomo and Kanai، نويسنده , , Kazutaka and Ito، نويسنده , , Naoyuki and Higuchi، نويسنده , , Seiichi and Hoshi، نويسنده , , Fumio، نويسنده ,
Abstract :
Full-length cDNA that encodes feline α1-microglobulin (Feα1m)-bikunin was obtained from a feline liver and cloned using an oligo-capping method. The Feα1m-bikunin cDNA was found to contain 1284 nucleotides, and Feα1m was found to include an open reading frame encoding a polypeptide of 201 amino acids. The deduced amino acid sequence of Feα1m showed varying amino acid identity when compared with the published sequences of the related α1-m of other species, ranging from 71.1 to 82.1%. Feα1m mRNA expression was confirmed by reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR analysis in the cerebrum, cerebellum, lung, heart, liver, spleen, pancreas, kidney, adrenal gland, and testicle. The highest Feα1m mRNA level was found in the liver.
Keywords :
?1-Microglobulin , CAT , CLONING , Expression
