Author/Authors :
Wang، نويسنده , , Lu and Zhang، نويسنده , , Wei and Wu، نويسنده , , Ding-An and Chen، نويسنده , , Chao and Xu، نويسنده , , Qin-Zi and Zhao، نويسنده , , Bo and Liao، نويسنده , , Yong-De and Zhao، نويسنده , , Shu-Hong and Fu، نويسنده , , Xiang-Ning and Chen، نويسنده , , Shi and Zhu، نويسنده , , Min، نويسنده ,
Abstract :
Nectin-2, also known as poliovirus receptor-related 2 or CD112, has been identified in many animals and plays a crucial role in cell recognition and adhesion. Here we report the identification of two porcine Nectin-2 (poNectin-2) isoforms. The open reading frame (ORF) of 1440 nucleotides (nt) of poNectin-2α encodes 479 amino acids (aa). poNectin-2δ gene consists of 1620 nt of ORF with 539 aa. The deduced aa sequences of poNectin-2α and poNectin-2δ gene exhibit high identity with human (74% and 79%) and mouse (71% and 76%) orthologs, respectively. The ectodomains of deduced poNectin-2 protein share the structural feature of mammalian Nectin-2, including a conserved cysteine skeleton important for the formation of the three-dimensional structure. RT-PCR analysis showed that the mRNA of both poNectin-2 isoforms was broadly expressed in various tissues and cells. poNectin-2 gene was mapped to chromosome 6q21. Information from this study will help to elucidate the Nectin-2 pathway in xenotransplant immunity.
Keywords :
Gene expression , Porcine , Nectin-2 , Poliovirus receptor
