Title of article :
Construction and immunogenicity of a DNA vaccine containing clumping factor A of Staphylococcus aureus and bovine IL18
Author/Authors :
Yin، نويسنده , , Rong-lan and Li، نويسنده , , Chang and Yang، نويسنده , , Zheng-tao and Zhang، نويسنده , , Yan-jing and Bai، نويسنده , , Wenlin and Li، نويسنده , , Xiao and Yin، نويسنده , , Rong-huan and Liu، نويسنده , , Hui and Liu، نويسنده , , Shan and Yang، نويسنده , , Qi and Cao، نويسنده , , Yongguo and Zhang، نويسنده , , Nai-sheng، نويسنده ,
Pages :
5
From page :
270
To page :
274
Abstract :
Selection of potent cytokine adjuvants is important for the development of Staphylococcus aureus DNA vaccines. Several potential cytokines have been proven to induce enhanced immune responses in animal models and clinical tests. There is still no reported use of IL18 as an adjuvant to design DNA vaccines against S. aureus. In this study, we cloned the main fibronectin binding protein gene (a fragment from clumping factor A, ClfA221–550) of S. aureus and bovine interleukin 18 (bIL18). Then recombinant plasmids were constructed based on the eukaryotic expression vector pVAX1 with or without bIL18. Indirect immunofluorescence assays in transfected HeLa cells indicated that the recombinant DNAs (rDNAs) could be expressed correctly and had antigenicity. BALB/c mice were used as experimental models to examine the immunogenicity of rDNAs in vivo. The ClfA221–550 rDNA provoked antibody production. The bIL18 rDNA induced production of the Th1 type cytokines IL2 and IFNγ, and ClfA221–550 and bIL18 synergistically stimulated T-lymphocyte proliferation. The data demonstrated that bIL18 is a potent adjuvant that could be used to enhance cellular immunity.
Keywords :
Staphylococcus aureus , bIL18 , CLFA , DNA vaccine
Journal title :
Astroparticle Physics
Record number :
2060151
Link To Document :
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