Author/Authors :
Hong، نويسنده , , Yeong Ho and Lillehoj، نويسنده , , Hyun S. and Lee، نويسنده , , Sung Hyen and Park، نويسنده , , Myeong-Sun and Min، نويسنده , , Wongi and LaBresh، نويسنده , , Joanna and Tompkins، نويسنده , , Dannielle and Baldwin، نويسنده , , Cynthia، نويسنده ,
Abstract :
Four mouse monoclonal antibodies (mAbs) which are specific for chicken interleukin 18 (chIL18) were produced and characterized by enzyme-linked immunosorbent assay (ELISA), Western blotting, quantitative real-time PCR and neutralization assays. Using Western blot analysis, monoclonal antibodies specific for chIL18 identified a 23 kDa Pichia pastoris-expressed chIL18 and 66 kDa E. coli-derived MBP fusion protein of chIL18. Bioassays for chIL18 using primary chicken spleen cells showed dose-dependent IFN-γ mRNA expression and induction of IFN-γ from primary splenocytes, and triggered nitric oxide (NO) production in the HD11 macrophage cell line. These mAbs showed neutralizing chIL18 activity. Taken together, these mouse mAbs which detect chicken IL-18 will be significant new immune reagents and useful tools for basic and applied research in poultry.
Keywords :
Interleukin 18 , monoclonal antibody , IL-18 , chicken
