Author/Authors :
Mochizuki، نويسنده , , Hiroyuki and Takahashi، نويسنده , , Masashi and Nishigaki، نويسنده , , Kazuo and Ide، نويسنده , , Tetsuya and Goto-Koshino، نويسنده , , Yuko and Watanabe، نويسنده , , Shinya and Sato، نويسنده , , Hirofumi and Sato، نويسنده , , Masahiko and Kotera، نويسنده , , Yukiko and Fujino، نويسنده , , Yasuhito and Ohno، نويسنده , , Koichi and Uchida، نويسنده , , Kazuyuki and Tsujimoto، نويسنده ,
Abstract :
We established a novel feline B-cell line, MS4, from the neoplastic pleural effusion of a cat with cutaneous B-cell lymphoma. Immunophenotype staining of the MS4 cells was positive for CD20, CD79α, and IgA and negative for CD3, CD4, CD5, CD8α, CD18, CD21, CD22, IgM, IgG, Ig light chain, and MHC class II. PCR analysis for immunoglobulin heavy chain gene rearrangements revealed a monoclonal rearrangement, whereas no clonal rearrangement of the T-cell receptor γ gene was detected. Southern blotting with an exogenous feline leukemia virus (FeLV) U3 probe revealed no integration of exogenous FeLV provirus. The MS4 cell line is the first FeLV-negative feline B-cell lymphoma cell line, and may be used to investigate the pathogenesis of spontaneously occurring feline lymphoma and the development of new therapies.
Keywords :
B-cell , Lymphoma , CAT , FeLV
