Author/Authors :
Umeki، نويسنده , , Saori and Suzuki، نويسنده , , Ryoichi and Shimojima، نويسنده , , Masayuki and Ema، نويسنده , , Yasuo and Yanase، نويسنده , , Takuma and Iwata، نويسنده , , Hiroyuki and Okuda، نويسنده , , Masaru and Mizuno، نويسنده , , Takuya، نويسنده ,
Abstract :
Thirteen different monoclonal antibodies against canine P-selectin glycoprotein ligand-1 (cPSGL-1) were obtained by immunization of rats with cells of a canine lymphoma cell line (Ema). O-sialoglycoprotein endopeptidase treatment of Ema cells showed that all of these antibodies recognized O-glycosylated peptides of canine PSGL-1. Experiments using deletion or point mutants of cPSGL-1 indicated that these antibodies could be categorized into several groups based on their cPSGL-1 recognition characteristics. These anti-cPSGL-1 monoclonal antibodies will be useful for analysis of the canine P-selectin and PSGL-1 system.
Keywords :
monoclonal antibody , Hybridoma , PSGL-1 , canine
