Author/Authors :
Li، نويسنده , , Yan and Jia، نويسنده , , Ying and Wen، نويسنده , , Kai and Liu، نويسنده , , Hua and Gao، نويسنده , , Mingchun and Ma، نويسنده , , Bo and Zhang، نويسنده , , Wenlong and Wang، نويسنده , , Junwei، نويسنده ,
Abstract :
Bovine viral diarrhea virus (BVDV) is a member of the genus Pestivirus within the family Flaviviridae. NS3 is one of the immunodominance regions of the BVDV viral proteins. To identify the potential B-cell linear antigenic epitopes within BVDV NS3 region, serial overlapping truncations covering the whole region were expressed and purified, and screened by multistep of Western-blot. We found (1VCKKITEHERCHVNI15), (20AFFGVMPRGTTPRAPVR36), (46RRGLETGWAYTHQGGI61), (281EGDMATGITYASYGYFC297), (426YSGEDPANLRVVTSQSPYVVVATNAIESGV455) and (481FIVTGLKRMAVTVGEQA497) can be recognized by the BVDV infected bovine serum. These proteins have been confirmed by indirect enzyme-linked immunosorbent assay (I-ELISA). The results of this study might open new perspectives on the structure and antibody–antigen reaction of the non-structural proteins and may aid in the clinical application as well.
