Title of article :
Feasibility assessment of in vitro chemoresponse assay on stereotactic biopsies of glioblastoma multiforms: a step towards personalized medicine
Author/Authors :
-، - نويسنده Health Management Department, Quality Assurance Deputy, Razi Vaccine & Serum Research Institute, Karaj, Iran Sadeghi Fazel, Fariba , -، - نويسنده Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Haddadi, Mahnaz , -، - نويسنده Department of Neurosurgery, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran Khoshnevisan, Alireza , -، - نويسنده Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran Muhammadnejad, Samad , -، - نويسنده Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Muhammadnejad, Ahad , -، - نويسنده Anatomy Department, School of Medicine, Tarbiat Modares University, Tehran, Iran Mazaheri, Zohreh , -، - نويسنده Omid Institute for Advance Biomodels, Incubation Center for Medical Devices, Tehran University of Medical Sciences, Tehran, Iran Arjomandnejad, Motahareh , -، - نويسنده Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Shirkoohi, Reza , -، - نويسنده Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran Oghabian, Mohammad-Ali , -، - نويسنده Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran Sherkat-Khameneh, Narjes , -، - نويسنده Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Amanpour, Saeid , -، - نويسنده Virology Department, Pasteur Institute of Iran, Tehran, Iran Kazemimanesh, Monireh
Issue Information :
فصلنامه با شماره پیاپی 0 سال 2014
Pages :
4
From page :
922
To page :
925
Abstract :
-
Abstract :
Objective(s):P In vitro chemosensitivity and resistance assays (CSRAs) are a promising tool for personalized treatment of glioblastoma multiform (GBM). These assays require a minimum of 1 to 2 g of tumor specimen for testing, but this amount is not always accessible. We aimed to assess the feasibility and validity of utilizing stereotactic biopsies of GBM in CSRAs. Materials and Methods: Single cell suspension was prepared from 1 g weight explants of the established xenograft tumor of GBM. Also, primary culture was carried out on 35 mg weight specimens, as a surrogate for stereotactic biopsies. Then, chemoresponse profile of cells obtained by direct cell disaggregation and primary culture was determined using temozolomide and carmustine by clonogenic assay[AGA1] . Results: There was no statistically significant difference in the cytotoxicity of temozolomide and carmustine between cells obtained from both methods. Conclusion: This work supports the feasibility of using stereotactic biopsies of GBM in CSRAs.
Journal title :
Iranian Journal of Basic Medical Sciences
Serial Year :
2014
Journal title :
Iranian Journal of Basic Medical Sciences
Record number :
2063040
Link To Document :
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