Title of article
Feasibility assessment of in vitro chemoresponse assay on stereotactic biopsies of glioblastoma multiforms: a step towards personalized medicine
Author/Authors
-، - نويسنده Health Management Department, Quality Assurance Deputy, Razi Vaccine & Serum Research Institute, Karaj, Iran Sadeghi Fazel, Fariba , -، - نويسنده Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Haddadi, Mahnaz , -، - نويسنده Department of Neurosurgery, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran Khoshnevisan, Alireza , -، - نويسنده Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran Muhammadnejad, Samad , -، - نويسنده Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Muhammadnejad, Ahad , -، - نويسنده Anatomy Department, School of Medicine, Tarbiat Modares University, Tehran, Iran Mazaheri, Zohreh , -، - نويسنده Omid Institute for Advance Biomodels, Incubation Center for Medical Devices, Tehran University of Medical Sciences, Tehran, Iran Arjomandnejad, Motahareh , -، - نويسنده Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Shirkoohi, Reza , -، - نويسنده Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran Oghabian, Mohammad-Ali , -، - نويسنده Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran Sherkat-Khameneh, Narjes , -، - نويسنده Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Amanpour, Saeid , -، - نويسنده Virology Department, Pasteur Institute of Iran, Tehran, Iran Kazemimanesh, Monireh
Issue Information
فصلنامه با شماره پیاپی 0 سال 2014
Pages
4
From page
922
To page
925
Abstract
-
Abstract
Objective(s):P In vitro chemosensitivity and resistance assays (CSRAs) are a promising tool for personalized treatment of glioblastoma multiform (GBM). These assays require a minimum of 1 to 2 g of tumor specimen for testing, but this amount is not always accessible. We aimed to assess the feasibility and validity of utilizing stereotactic biopsies of GBM in CSRAs. Materials and Methods: Single cell suspension was prepared from 1 g weight explants of the established xenograft tumor of GBM. Also, primary culture was carried out on 35 mg weight specimens, as a surrogate for stereotactic biopsies. Then, chemoresponse profile of cells obtained by direct cell disaggregation and primary culture was determined using temozolomide and carmustine by clonogenic assay[AGA1] . Results: There was no statistically significant difference in the cytotoxicity of temozolomide and carmustine between cells obtained from both methods. Conclusion: This work supports the feasibility of using stereotactic biopsies of GBM in CSRAs.
Journal title
Iranian Journal of Basic Medical Sciences
Serial Year
2014
Journal title
Iranian Journal of Basic Medical Sciences
Record number
2063040
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