Author/Authors :
نوين، معرفت غفاري نويسنده Dept. of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Velenjak, Novin, Marefat Ghaffari , نوروزنيا، مهرداد نويسنده PhD ژنتيك پزشكي ـ استاديار دانشكده پزشكي دانشگاه تربيت مدرس ـ تهران ـ ايران Norouz Nia, .M , الله ويسي، عذرا نويسنده Dept. of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Velenjak, Tehran Allahveisi, Azra , صارمي ، ابوطالب نويسنده , , فتح آبادي، فاطمه فدايي نويسنده Dept. of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Velenjak, Tehran Fathabadi, Fateme Fadaei , فراهاني ، رضا نويسنده , , فرد، علي نويسنده , , پولادي، آرش نويسنده pouladi, arash , فرد، رامين مظاهري نژاد نويسنده Rastegar Central Laboratory, Faculty of Veterinary Medicine, University of Tehran, Tehran Fard, Ramin Mazaherinezhad , يوسفيان، الهام نويسنده دانشگاه شهيد بهشتي Yoosefian, E
Abstract :
Background: The aim of the current study was to assess the mRNA levels of two mitochondria-related genes, including nuclear-encoded NRF1 (nuclear respiratory factor 1), mitochondrial transcription factor A (TFAM), and mitochondrial-encoded cytochrome c oxidase subunit 1 (MT-CO1) genes in various stages of the human oocyte maturation. Methods: Oocytes were obtained from nine infertile women with male factor undergoing in vitro fertilization (IVF)/intra-cytoplasmic sperm injection protocol. Mitochondrial-related mRNA levels were performed by single-cell TaqMan real-time PCR. Results: the expression level of the target genes was low at the germinal vesicle stage (P > 0.05). Although the mRNA level of NRF1gene remained stable in metaphase I, the mRNA level of TFAM and MT-CO1 increased significantly (P < 0.05).In metaphase II, the expression level of all genes increased compared to metaphase I (P < 0.05). Conclusion: The overexpression levels of NRF1, TFAM, and MT-CO1 genes are related to the oocyte maturation. Therefore, the current study could be used clinically to improve the success rate of IVF. Iran. Biomed. J. 19 (1): 23-28, 2015
