Author/Authors :
-، - نويسنده Department of Biotechnology, Iranian Research Organization for Science and Technology, P.O. Box 15815-3538 Tehran, I.R. IRAN Rostami, Khosrow , -، - نويسنده Mork Family Department of Chemical Engineering and Materials Science, University of South California,
925 Bloom Walk, HED 216, Los Angeles, CA 90089-1211, USA Dayani, Yasaman , -، - نويسنده Department of Biotechnology, Iranian Research Organization for Science and Technology, P.O. Box 15815-3538 Tehran, I.R. IRAN Esfahani Bolandbalaie, Zahra , -، - نويسنده Faculty of Chemical & Petroleum Engineering, Sharif University of Technology, P.O. Box 1365-8639, Tehran I.R. IRAN Rashtchian, Davood
Abstract :
The present work examines leaching of lipoxygenase-1 from soybean flour employing stirred tank vessel. The effect of operating parameters, impellers speed, operational period, temperature, pH and scale-up were considered.The acetic acid pH: 5.2 increased the leaching of lipoxygenase- 1.The sensitivity of agitator speed and geometrical-scale-up on the enzymeleaching has been conducted. The effect of agitator speed on the geometrically- scaled- up reactor has shown that similar amount of enzyme is leached at lower speed. The result is in para with the conventional system. The importance of operational period from 5 to 50 min on the enzyme leaching was evaluated and higher enzyme leaching obtained within 10 min. of operation. The Sigmaplot-6 for statistical verification and developing a correlation for the enzyme leaching was used.
