Molecular Typing of Salmonella Isolates in Poultry by Pulsed-Field Gel Electrophoresis in Iran

Salmonella is one of the most widespread zoonotic enter pathogenic microorganisms found in the global food chain. Poultry and Poultry products have been identified as one of the important foodborne sources of Salmonella. Pulsed-Field Gel Electrophoresis (PFGE) is a gold standard typing method for identification of Salmonella isolates during outbreaks and epidemiological investigations. The aim of this study was to carry out molecular typing of Salmonella enterica spp. by PFGE technique. All 47 Salmonella isolates were serotyped and then subjected to PFGE. Total isolates were analyzed by means of the molecular technique XbaI PFGE. In the current work, PFGE and serotyping were used to subtype 47 Salmonella isolates belonging to 22 different serotypes and derived from poultry. Thirty-nine PFGE patterns out of 47 isolates were obtained. The Discrimination Index (DI) by serotyping (0.93) was lower than PFGE (DI = 0.99). In conclusion, molecular methods such as PFGE can be used for epidemiological characterization of Salmonella serotypes.

Salmonella is one of the most widespread zoonotic enter pathogenic microorganisms found in the global food chain. Poultry and Poultry products have been identified as one of the important foodborne sources of Salmonella. Pulsed-Field Gel Electrophoresis (PFGE) is a gold standard typing method for identification of Salmonella isolates during outbreaks and epidemiological investigations. The aim of this study was to carry out molecular typing of Salmonella enterica spp. by PFGE technique. All 47 Salmonella isolates were serotyped and then subjected to PFGE. Total isolates were analyzed by means of the molecular technique XbaI PFGE. In the current work, PFGE and serotyping were used to subtype 47 Salmonella isolates belonging to 22 different serotypes and derived from poultry. Thirty-nine PFGE patterns out of 47 isolates were obtained. The Discrimination Index (DI) by serotyping (0.93) was lower than PFGE (DI = 0.99). In conclusion, molecular methods such as PFGE can be used for epidemiological characterization of Salmonella serotypes.