Study of Compatibility Relationships Among Some Almond Cultivars and Genotypes Using of SAlleles Identification

Almond (Prunus dulcis L.) is one of the most important nut crops in Iran. Most almond cultivars and genotypes are self-incompatible. However, research on S-alleles indicates that it is very efficient in cultivar selection. Self-incompatibility in almond is gametophytic and controlled by a single S-locus with multiple codominant alleles. In this study, compatibility relationships among cultivars, “Tuono”, “Shokofeh”, “Sahand” and five improved genotypes “A_1.16”, “A_9.7”, “A_8.39”,“A_10.11” and “A₂₃₀,” was investigated by the PCR of S-alleles. Degenerate primers (PaConsI-F, EM-PC1consRD, EM-PC2consFD, and EM-PC3consRD) were used for amplification of S-alleles. Results showed that only “A_10.11” and “A_8.39” were completely cross-incompatible, but all of the other studied cultivars and genotypes were cross-compatible. Furthermore, cultivar “Tuono” and genotype “A_1.16” had a self-fertility allele.

Almond (Prunus dulcis L.) is one of the most important nut crops in Iran. Most almond cultivars and genotypes are self-incompatible. However, research on S-alleles indicates that it is very efficient in cultivar selection. Self-incompatibility in almond is gametophytic and controlled by a single S-locus with multiple codominant alleles. In this study, compatibility relationships among cultivars, “Tuono”, “Shokofeh”, “Sahand” and five improved genotypes “A_1.16”, “A_9.7”, “A_8.39”,“A_10.11” and “A₂₃₀,” was investigated by the PCR of S-alleles. Degenerate primers (PaConsI-F, EM-PC1consRD, EM-PC2consFD, and EM-PC3consRD) were used for amplification of S-alleles. Results showed that only “A_10.11” and “A_8.39” were completely cross-incompatible, but all of the other studied cultivars and genotypes were cross-compatible. Furthermore, cultivar “Tuono” and genotype “A_1.16” had a self-fertility allele.