Biosensing effects in functionalized electroconducting conjugated polymer layers: addressable DNA matrix for the detection of gene mutations

A new methodology for the preparation of addressed DNA matrices is described. The process is based on a one-step electrodeposition of a polypyrrole film functionalized by a covalently linked oligonucleotide. Even in the bulk of the film the single-stranded DNA fragment can be hybridized or denatured. A support bearing four oligonucleotide spots was obtained by successive copolymerization on a four electrode matrix. This device allows detection of three ras mutations on a synthetic DNA fragment without cross contamination.