Electrochemical and sensing properties of enzyme-polypyrrole multicomponent electrodes

A structurally well-defined multilayer enzyme electrode comprised of a lipid-modified glucose oxidase and an electrically conductive poly(pyrrole) LB film for detecting glucose was fabricated by using the Langmuir-Blodgett technique. The voltammetric measurements of these electrodes revealed that the electrically conductive poly(pyrrole) layers could quantitatively detect the hydrogen peroxide produced by the modified enzyme layers. The amperometric anodic current (at 0.6 V vs. Ag/AgCl) of the electrode increased with increasing the glucose concentration. A linear response to glucose concentration was observed in the range of 15–100 mM with a detection limit of 5 mM.