Author/Authors :
Lu، نويسنده , , Wenbo and Luo، نويسنده , , Yonglan and Chang، نويسنده , , Guohui and Qin، نويسنده , , Xiaoyun and Liao، نويسنده , , Fang and Sun، نويسنده , , Xuping، نويسنده ,
Abstract :
In the present study, we demonstrate the first use of polypyrrole colloidal nanospheres (PPyCNPs) as an effective sensing platform for fluorescence-enhanced DNA detection by the following two steps: (1) PPyCNP adsorbs and quenches a dye-labeled single-stranded DNA (ssDNA) probe; (2) The subsequent hybridization of the probe with its target produces a double-stranded DNA (dsDNA) which detaches from PPyCNP, leading to recovery of dye fluorescence. The present system shows a detection limit as low as 1 nM and has a high selectivity down to single-base mismatch.
