Molecular assembly of multilayer enzyme: toward the development of a chemiluminescence-based fiber optic biosensor

We report here on a technique to immobilize a multilayer enzyme assembly on an optical fiber surface. A multilayer of an enzyme, alkaline phosphatase, was immobilized by chemical cross-linking on an optical fiber surface. Chemiluminescence, ellipsometry, and surface plasmon resonance were used to characterize the structure and activity of the assembly. A chemiluminescence-based fiber optic biosensor utilizing this immobilization technique has been developed for the detection of organophosphorus-based pesticides. Detection of pesticide at sub-ppm level has been achieved for paraoxon.