Cytocompatibility of new Ti alloy without Al and V by evaluating the relative growth ratios of fibroblasts L929 and osteoblasts MC3T3-E1 cells

The cytocompatibility of new Ti alloys not containing Al and V such as Ti-15Zr-4Nb-4Ta-0.2Pd-0,05%N-0.2%O and Ti-15Sn-4Nb-2Ta-0.2Pd-0.2%O were investigated by comparing with those of pure Ti grade 2 andTi-6%Al-4%V ELI (extra low interstitial) alloys. The relative growth ratios of both L929 and MC3T3-E1 cells for the new Ti alloys were almost equal to 1 and were slightly higher than pure Ti grade 2 and Ti-6Al-4V alloys. Also, the influence of extracting conditions with E-MEM solution and ultrapure water on the relative growth ratio of cells were examined. Furthermore, the relationship between metallic concentration in the medium extracted with Ti, Al, V, Zr, Sn, Nb, Ta and Pd powders and the relative growth ratios of L929 cells were evaluated. The relative growth ratio for the medium extraction of Al, containing very little V ions (less than 0.02 ppm) tend to be too low as compared to that of V free Al extraction. However, extraction of Ti, Zr, Sn, Nb and Ta powders, and mixtures of powders such as Ti + Zr + Mb + Ta and Ti + Sn + Nb + Ta had evidently no effect on the relative growth ratio of L929 cells.