Immobilization of glucose oxidase in sol–gel matrix and its application to fabricate chemiluminescent glucose sensor

In this paper, glucose oxidase (GOD) was immobilized in the course of sol–gel process of TEOS, and we cast the GOD-doped sol to different supports (silica gel and molecular sieve) to prepare the GOD columns. It was demonstrated that the activity of GOD remained high in sol–gel matrix. With the application of the GOD column prepared with sol–gel method to a flowing injection chemiluminescent system, glucose could be effectively determined with the linear range of the concentration in 3.5–70 μmol/l and the detection limit was at 0.6 μmol/l.