Effect of mechanical stretch on TGF-β1 expression of C2C12 myogenic cells

The purpose of this study was to examine whether mechanical stretch altered the expression of transforming growth factor (TGF)-β1 in cultured myogenic satellite cell. C2C12 myogenic satellite cell was plated on collagenized silastic membrane and cultured in growth medium and stretched 20% elongation for 24 h. The expression of TGF-β1 was determined by semiquantitive reverse transcriptional polymerase chain reaction (RT-PCR) analysis. Mechanical stretch decreased myogenic regulatory factors. The expression of TGF-β1 was increased in stretched C2C12 as compared to control. This result demonstrates that mechanical stretch inhibits myogenic differentiation by an increase of TGF-β1 expression on C2C12 myogenic satellite cell.