Application of polyaniline-Langmuir-Blodgett films as a glucose biosensor

Langmuir-Blodgett films of the polyemeraldine base form of polyaniline (PANI) have been successfully deposited on an indium tin oxide (ITO) coated glass surface. The enzyme glucose oxidase (GOX) was entrapped between the layers of the Langmuir-Blodgett (LB) film. The immobilized GOX was tested for retention of its catalytic activity and its ability to perform like a reagentless glucose biosensor. The electroactivity of such PANI-LB-GOX electrodes was tested using cyclic voltammetry (CV). On the basis of the anodic peak current at 0.7 V with respect to Ag/AgCl, a calibration curve for a PANI-LB-GOX based glucose biosensor was obtained.