Cultured oral epithelium as an effective biological dressing using for palatal wounds after palatoplasty

Cultured allografts derived from mucosal tissue provide a potent stimulus to wound healing in a wide variety of wounds. field of oral surgery, mucoperiosteal defect of the hard palate after palatoplasty causes scar contraction, leading to poor growth of the maxilla. The promotion of wound healing in these cases through cultured epithelial allografting has been reported. Cultured epithelial allografting was done using a strangerʹs cultured cells. We grafted cultured oral epithelium in the hope of improving growth of maxilla. of the soft and hard palate (seven patients), and a cleft of the soft palate (two patients) were present. Average patient age was 1 year 4 months. Palatoplasty was done by a conventional pushback operation. Oral epithelial cells in healthy adults were cultured using 3T3 cells as the feeder layer. After 3 weeks, cultured oral mucosal epithelium was grafted on a raw surface following palatoplasty. patients, the grafted areas underwent re-epithelialization after about 1 week and did not exhibit any side effects of graft rejection. Grafted areas healed completely after 2–3 weeks in all cases. ed epithelial allografts serve as a temporary biological dressing, and accelerates epithelialization and wound healing. Allografting by cultured oral epithelium has proved to be a very useful therapeutic modality in palatoplasty, as well as effective augmentation materials in cases of oral mucosal defects.