Amphiphilic polymer–liposome interaction: a novel immobilization technique for liposome on gel surface

We developed a simple method for the immobilization of liposomes and biological membrane fragments to gel surface using an amphiphilic polymer, consisting of octadecylacrylamide and N,N-dimethylacrylamide with a molar ratio of 99:1. Calcein-entrapped egg phosphatidylcholine liposomes were successfully immobilized to the surface of polyacrylamide gel containing the polymer without significant loss of entrapped calcein. Different contents of the amphiphilic polymer in the gel resulted in the different surface density of immobilized liposomes on the surface of the gel in a dose-dependent manner. As an example of biological membranes, microsome membranes from yeast cells were also well immobilized to the gel.