Micropatterning of diacetylenic liposomes on glass surfaces

We report the results on the immobilization of polydiacetylene liposomes on glass substrates obtained by the use of micro-contact printing (μCP) technique. Micro-patterned self-assembled monolayer of 3-aminopropyltriethoxysilane (APS) was formed at contact regions of a polydimethylsiloxane (PDMS) stamp with the clean glass surfaces. Polydiacetylene liposomes were then covalently attached to the APS regions, by amidization between surface amine groups of the pattern and N-hydroxy succinimide moieties of the liposomes. Fluorescence images revealed that the polydiacetylene liposomes were successfully immobilized on glasses forming patterns of squares with a width of 70 μm and dots with diameters of 15 to 50 μm. The results would be potentially useful for developing stable liposome-array sensors based on glasses.