Carbon paste biosensor for phenol detection of impregnated tissue: modification of selectivity by using β-cyclodextrin-containing PVA membrane

In order to detect and quantify with a biosensor the phenol contents in animal tissue (salmon flesh) by simple contact, we have undertaken experimental study on nonwoven cellulose fibers soaked with phenolic compounds. An electrochemical biosensor made of carbon paste and tyrosinase mixed together with electropolymerized pyrrole was elaborated. Phenol detection was realized by electrochemical reduction of quinones produced by the tyrosinase activity. The biosensor was first optimized based on enzyme loading and nature of the carbon paste. A semipermeable membrane containing cyclodextrin moieties was deposited on the biosensor in order to test its sensitivity for phenol detection. Finally, the biosensor was put in contact with phenol absorbing cellulose fibers. Results showed that the relaxation time response of the sensor was relevant of phenol concentration.