Biopolymer-clay nanoparticles composite system (Chitosan-laponite) for electrochemical sensing based on glucose oxidase

Nanocomposite matrix based on chitosan/laponite was successfully utilized to construct a new type of amperometric glucose biosensor. This hybrid material combined the merits of organic biopolymer, chitosan, and synthesized inorganic clay, laponite. Glucose oxidase (GOD) immobilized in the material maintained its activity well as the usage of glutaraldehyde was avoided. The composite films were characterized by Fourier transform infrared (FT-IR). The parameters affecting the fabrication and experimental conditions of biosensors were optimized. The sensitivity of the proposed biosensor (33.9 mA M− 1 cm− 2) permitted the determination of glucose in the concentration range of 1 × 10− 6–5 × 10− 5 M with a detection limit of 0.3 μM based on S/N = 3. The apparent Michaelis–Menten constant (KMapp) for the sensor was found to be 15.8 mM.