Title of article :
Microcontact imprinted surface plasmon resonance sensor for myoglobin detection
Author/Authors :
Osman، نويسنده , , Bilgen and Uzun، نويسنده , , Lokman and Be?irli، نويسنده , , Necati and Denizli، نويسنده , , Adil، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2013
Pages :
6
From page :
3609
To page :
3614
Abstract :
In this study, we prepared surface plasmon resonance (SPR) sensor using the molecular imprinting technique for myoglobin detection in human serum. For this purpose, we synthesized myoglobin imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-l-tryptophan methyl ester) [poly(HEMA-MATrp)] nanofilm on the surface of SPR sensor. We also synthesized non-imprinted poly(HEMA-MATrp) nanofilm without myoglobin for the control experiments. The SPR sensor was characterized with contact angle measurements, atomic force microscopy, X-ray photoelectron spectroscopy, and ellipsometry. We investigated the effectiveness of the sensor using the SPR system. We evaluated the ability of SPR sensor to sense myoglobin with myoglobin solutions (pH 7.4, phosphate buffer) in different concentration range and in the serum taken from a patient with acute myocardial infarction. We found that the Langmuir adsorption model was the most suitable for the sensor system. The detection limit was 87.6 ng/mL. In order to show the selectivity of the SPR sensor, we investigated the competitive detection of myoglobin, lysozyme, cytochrome c and bovine serum albumin. The results showed that the SPR sensor has high selectivity and sensitivity for myoglobin.
Keywords :
Molecularly imprinted polymer , Microcontact imprinting , SPR sensor , nanofilm , myoglobin
Journal title :
Materials Science and Engineering C
Serial Year :
2013
Journal title :
Materials Science and Engineering C
Record number :
2103369
Link To Document :
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