Title of article :
In vitro evaluation of osteoconductivity and cellular response of zirconia and alumina based ceramics
Author/Authors :
Pandey، نويسنده , , Ajoy Kumar and Pati، نويسنده , , Falguni and Mandal، نويسنده , , Debika and Dhara، نويسنده , , Santanu and Biswas، نويسنده , , Koushik، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2013
Pages :
8
From page :
3923
To page :
3930
Abstract :
Developed ceria/yttria stabilized zirconia and ceria/yttria stabilized zirconia toughened alumina supported formation of apatite layer when immersed in simulated body fluid without any prior surface treatment. The formed mineral layer was confirmed as hydroxyapatite through X-ray diffraction patterns. The calcium/phosphate atomic ratio obtained from energy dispersive X-ray spectroscopy was found to be little less (Ca/P = 1.5) than that of pure hydroxyapatite (Ca/P = 1.7) which indicates the probability of mixed type calcium-phosphate compound formation. The achieved thickness of apatite layer was estimated through a surface profilometer and as high as ~ 17 μm thickness was found after 28 days of soaking. The biocompatibility of the developed materials was ensured through in vitro human osteoblast like cell (MG63) culture on ceramic discs. The morphology of attached cells was characterized through scanning electron microscopy and fluorescent microscopy which show multilayered interconnected cell growth within 8 days of culture period. Moreover, differentiation of MG63 cells was evaluated through MTT assay, total protein content and alkaline phosphatase activity.
Keywords :
Bio-ceramic , Osteoconduction , In vitro biocompatibility , Cell culture , Bioactivity
Journal title :
Materials Science and Engineering C
Serial Year :
2013
Journal title :
Materials Science and Engineering C
Record number :
2103455
Link To Document :
بازگشت