Establishment and characterization of a pronephric stromal cell line (TPS) from rainbow trout,Oncorhynchus mykissW.

A new stromal adherent cell line, called trout pronephric stroma (TPS), was initiated from a long-term pronephric culture of an adult rainbow trout, and subcultured 104 times over a period of 4 years. This study describes the culture conditions and characterization by enzyme-cytochemistry, electron microscopy, isoenzyme profile, cytogenetic techniques, and viral susceptibility. The cell types in TPS cultures consisted of fibroblastic, epithelioid and giant cells. The modal chromosome number is 58. The optimum growth temperature of the TPS cells is 18-22°C, with a population doubling time of 27 h. The TPS line is susceptible to viral haemorrhagic septicaemia (VHS) virus and infectious haemopoietic necrosis (IHN) virus.