Title of article :
Molecular cloning of carp (Cyprinus carpio) C-type lectin and pentraxin by use of suppression subtractive hybridisation
Author/Authors :
Fujiki، نويسنده , , Kazuhiro and Bayne، نويسنده , , Christopher J. and Shin، نويسنده , , Dong-Ho and Nakao، نويسنده , , Miki and Yano، نويسنده , , Tomoki، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2001
Pages :
5
From page :
275
To page :
279
Abstract :
Fish β-galactoside binding lectin (galectin) cDNA was cloned from the cDNA library of rainbow trout (Oncorhynchus mykiss) head kidney. The clone contained a single open reading frame encoding 341 amino acids (aa) (38 kDa protein), including the initiator methionine. Significant sequence homology to mammalian galectin-9 (40–55% identity) was observed. Its amino acid sequence showed two distinct N- and C-terminal domains (148 and 130 aa, respectively) connected by a peptide linker (63 aa). The galectin contains two consensus WG-E-R/K motifs thought to play an essential role in sugar-binding, indicating that this lectin is a member of the tandem-repeat type galectins which have not been identified in fish. The 1·6 kDa mRNA of the lectin was found by Northern blot analyses to be widely expressed in the spleen, head kidney, thymus, peritoneal exudate cells, ovary, gills and heart. Southern blot analyses with the probe for C-terminal of the lectin showed the existence of two hybridising genes. These results suggest that rainbow trout has at least one tandem-repeat type galectin as well as proto-type galectin.
Keywords :
pentraxin , Suppression subtractive hybridisation , Teleost , C-type lectin , Carp , acute phase reactant
Journal title :
Fish and Shellfish Immunology
Serial Year :
2001
Journal title :
Fish and Shellfish Immunology
Record number :
2106634
Link To Document :
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