The typing of botulinal neurotoxins

The serological identification of Clostridium botulinum neurotoxin (BoNT) subtypes has shown to be elusive when current standard serologic tests are used. Based on (1) the in vivo response expected on quantitative BoNT-antitoxin systems and (2) the actual and the hypothetical antigenic makeup of BoNT subtypes, a comprehensive method for BoNTs typing is proposed.