Improvement of Salmonella detection on motility enrichment media by ferrioxamine E-supplementation of pre-enrichment culture

Supplementation of buffered peptone water with ferrioxamine E in concentrations ranging from 0.001 to 1.0 μg/ml significantly increased the motility of Salmonella on MSRV and DIASSALM semi-solid enrichment media. Zone diameters of swarming S. enteritidis and S. typhimurium increased more than twofold following use of pre-enrichment cultures supplemented with 0.01 μg ferrioxamine E/ml. The activity of ferrioxamine E is similar at 37 °C and 42 °C. Pre-enrichment of Salmonella in a variety of foods in supplemented buffered peptone water, with shaking at 37 °C for 6 h and motility enrichment at 42 °C for 16 h, enabled motile Salmonella to be detected in 1 day.