Title of article :
TRAIL in the mandarin fish Siniperca chuatsi: Gene and its apoptotic effect in HeLa cells
Author/Authors :
Gao، نويسنده , , Y. and Chang، نويسنده , , M.X. and Sun، نويسنده , , B.J. and Nie، نويسنده , , P.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2008
Pages :
12
From page :
55
To page :
66
Abstract :
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is one of the TNF superfamily members, participating in many biological processes including cell proliferation and apoptotic death. In this study, a TRAIL gene was cloned from a perciform fish, the mandarin fish Siniperca chuatsi, a major cultured fish in Chinaʹs aquaculture, and is named as SCTRAIL for S. chuatsi TRAIL. The full-length cDNA of SCTRAIL is 1359 bp, encoding a 283-amino-acid protein. This deduced protein contains the Cys231, a 23-mer fragment of transmembrane region, a glycosylation site and a TNF family signature, all of which are conserved among TRAIL members. SCTRAIL gene consists of six exons, with five intervening introns, spaced over approximately 9 kb of genomic sequence. Southern blotting demonstrated that the SCTRAIL gene is present as a single copy in mandarin fish genome. A 620 bp promoter region obtained by genome walking contains a number of putative transcription factor binding sites, such as Oct-1, Sp-1, NF-1, RAP-1, C/EBPalp, NF-κB and AP-1. The SCTRAIL is constitutively expressed in all the analyzed tissues, as revealed by RT-PCR, which is confirmed by Western blotting analysis using polyclonal antibody against bacteria-derived recombinant SCTRAIL protein. As an apoptosis-inducing ligand, the overexpression of SCTRAIL but not the mutant SCTRAIL-C203S in HeLa cells induced changes characteristic of apoptosis, including chromatin condensation, nucleus fragmentation, DNA ladder, and increase of sub-G0/G1 cells in FACS analysis.
Keywords :
Mandarin fish , apoptosis , Overexpression , Hela cells , Siniperca chuatsi , TRAIL
Journal title :
Fish and Shellfish Immunology
Serial Year :
2008
Journal title :
Fish and Shellfish Immunology
Record number :
2107915
Link To Document :
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