Title of article :
The development of a combined surface adhesion and polymerase chain reaction technique in the rapid detection of Listeria monocytogenes in meat and poultry
Author/Authors :
Duffy، نويسنده , , Geraldine and Cloak، نويسنده , , Orla M and Sheridan، نويسنده , , J.J. and Blair، نويسنده , , I.S and McDowell، نويسنده , , D.A، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1999
Abstract :
A procedure combining enrichment surface adhesion and polymerase chain detection (SA-PCR) was developed and applied in the detection of Listeria monocytogenes in meat products. Minced beef samples were inoculated with L. monocytogenes (log103 cfu g−1) and incubated for 10 h at 30°C in buffered peptone water. L. monocytogenes was recovered from the culture by attachment to a polycarbonate membrane immersed for 15 min in the enriched meat culture. The membrane and attached bacteria were removed from the culture and the membrane dissolved in phenol:chloroform. The DNA was extracted from the bacteria and a PCR assay was carried out using primers directed against the listerolysin O gene of L. monocytogenes. The combined (SA-PCR) technique had a detection limit of log10 4.0 cfu ml−1 in enriched meat cultures.The rapid technique was applied to a small number of retail samples (n=100) and was found to compare favourably to the standard cultural method. A total of 12 samples were confirmed positive for L. monocytogenes using the standard cultural method and the SA-PCR assay. No false positives or negatives were recorded by either method.
Keywords :
listeria , PCR , Surface adhesion
Journal title :
International Journal of Food Microbiology
Journal title :
International Journal of Food Microbiology