Title of article :
Differential gene expression profiles in Japanese flounder (Paralichthys olivaceus) with different susceptibilities to edwardsiellosis
Author/Authors :
Yasuike، نويسنده , , Motoshige and Takano، نويسنده , , Tomokazu and Kondo، نويسنده , , Hidehiro and Hirono، نويسنده , , Ikuo and Aoki، نويسنده , , Takashi، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2010
Pages :
6
From page :
747
To page :
752
Abstract :
Edwardsiellosis, which is caused by Edwardsiella tarda, a Gram-negative bacterium, is one of the most serious problems in Japanese flounder (Paralichthys olivaceus) culture. In this study, we examined the immune responses at the molecular level of genetic groups of Japanese flounder that are either susceptible or moderately susceptible to E. tarda infection using a cDNA microarray which was spotted with approximately 2000 different genes. Four different genetic groups of Japanese flounder (groups A, B, C and D) were infected with E. tarda by immersion. Mortality was 100% in groups A and C but only about 50% in groups B and D. Microarray analyses revealed 36 genes that were differentially expressed between the susceptible (A and C) and resistant (B and D) groups before E. tarda infection. Three days after the challenge, the resistant groups highly expressed MHC class I antigen processing and presentation–related genes, while the susceptible groups highly expressed genes involved in innate immune responses. The microarray results could be useful for selective breeding to enhance disease resistance of Japanese flounder against E. tarda and for studying strategies for controlling edwardsiellosis.
Keywords :
Edwardsiellosis , disease resistance , Microarray , Immune system , Japanese flounder
Journal title :
Fish and Shellfish Immunology
Serial Year :
2010
Journal title :
Fish and Shellfish Immunology
Record number :
2109357
Link To Document :
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