Title of article :
Antimicrobial and antioxidative enrichment of oak (Quercus robur) bark by rotation planar extraction using ExtraChrom®
Author/Authors :
Andren?ek، نويسنده , , Samo and Simonovska، نويسنده , , Breda and Vovk، نويسنده , , Irena and Fyhrquist، نويسنده , , Pia and Vuorela، نويسنده , , Heikki and Vuorela، نويسنده , , Pia، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Abstract :
The multifunctional ExtraChrom® instrument was used in the extraction of antimicrobial and radical scavenging components from oak (Quercus robur L.) bark. Milled and sieved oak bark was extracted with 80% (v/v) methanol solution in water on the ExtraChrom® instrument using step-gradient in the preparative separation. Extracts were tested using agar diffusion method on Staphylococcus aureus, Enterobacter aerogenes and Candida albicans. Some extracts showed moderate bactericidal, fungicidal, bacteriostatic and fungistatic activity. The composition related to activity of the fractions and extracts was screened simultaneously by thin-layer chromatography (TLC) detected by UV and by spraying the plate with radical scavenging reagent 1,1-diphenyl-2-picrylhydrazyl (DPPH) to detect antioxidant activity. Thus, we could demonstrate the antiradical and antimicrobial activity of oak beneficial in the storage of wine against the oxidation and human microbial exposure.
Keywords :
Quercus robur L. cortex , Rotation planar extraction , Staphylococcus aureus , Candida albicans , Enterobacter aerogenes , Radical scavenging activity , Thin-Layer Chromatography
Journal title :
International Journal of Food Microbiology
Journal title :
International Journal of Food Microbiology