Title of article :
Anti-metastasis effects of gallic acid on gastric cancer cells involves inhibition of NF-κB activity and downregulation of PI3K/AKT/small GTPase signals
Author/Authors :
Ho، نويسنده , , Hsieh-Hsun and Chang، نويسنده , , Chi-Sen and Ho، نويسنده , , Wei-Chi and Liao، نويسنده , , Sheng-You and Wu، نويسنده , , Cheng-Hsun and Wang، نويسنده , , Chau-Jong، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2010
Pages :
9
From page :
2508
To page :
2516
Abstract :
Polyphenols are natural antioxidants that are thought to contribute to prevention of cardiovascular disease and malignancy. Although many studies have been carried out to investigate the chemopreventive role of flavonoids, less attention has been focused on phenolic acids. In this study, the aim was to investigate the effect of phenolic acids found abundantly in vegetables, i.e. gallic acid (GA), caffeic acid (CA) and protocatechuic acid (PCA), on the inhibition of gastric adenocarcinoma (AGS) cell metastasis. The results showed 0.01 mM GA induced the same level of cell toxicity as 4.0 mM PCA. Using wound-healing assay and Boyden chamber assay, GA had potent inhibitory effects on AGS cell migration. The expression of MMP-2/9 of AGS cells was inhibited by 2.0 μM of GA. It is possible that the suppressive effect of GA on MMP-2/9 might involve the inhibition of NF-κB activity. Multiple proteins involved in metastasis and the cytoskeletal reorganization signal pathway, including Ras, Cdc42, Rac1, RhoA, RhoB, PI3K and p38MAPK, were also inhibited by GA. Furthermore, immunoreactivity assay of cytoskeletal F-actin demonstrated a significant inhibitory effect of GA treatment. In conclusion, GA may have the potential to be an effective agent for prevention and treatment of gastric cancer metastasis.
Keywords :
Gastric cancer , metastasis , PI3K/AKT , Gallic acid , MMP-2/9 , phenolic acid
Journal title :
Food and Chemical Toxicology
Serial Year :
2010
Journal title :
Food and Chemical Toxicology
Record number :
2114645
Link To Document :
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