Simultaneous determination of acrolein, malonaldehyde and 4-Hydroxy-2-Nonenal produced from lipids oxidized with fentonʹs reagent

Ethyl linoleate, ethyl linolenate, ethyl arachidonate and cod liver oil were oxidized with Fentonʹs reagent. Acrolein, malonaldehyde and 4-hydroxynonenal formed were derivatized to N-methylpyrazoline, N-methylpyrazole and 5-(1′-hydroxyhexyl)-l-methyl-2-pyrazoline with N-methylhydrazine, respectively. The derivatives were simultaneously analysed by gas chromatograph equipped with a fused silica capillary column and a nitrogen-phosphorus detector. The maximum amounts of acrolein (9.7 ± 2.11 nmol/ml) and malonaldehyde (61.18 ± 6.51 nmol/ml) were formed from cod liver oil. The highest amount of 4-hydroxynonenal (6.83 ± 0.53 nmol/ml) was produced from ethyl arathidonate.