Action of Methanolic Extract of Mung Bean Hulls as Inhibitors of Lipid Peroxidation and Non-lipid Oxidative Damage

The antioxidant effects of methanolic extract of mung bean hulls (MEMBH) on lipids and non-lipids, including liposome, carbohydrate, protein and 2′-deoxyguanosine (2′-dG), were investigated. MEMBH exhibited a remarkable antioxidant effect in a liposome model system, indicating that the extract was an inhibitor of lipid peroxidation. The inhibitory effect of MEMBH on deoxyribose damage was amount-dependent and it afforded considerable protection against damage to deoxyribose. In addition, MEMBH at low amounts was more effective in protecting protein oxidation. Furthermore, the oxidation of 2′-dG to 8-hydroxy-2′-deoxyguanosine (8-OH-2′dG) was inhibited by MEMBH. These results show that the extract also was an inhibitor of non-lipid oxidation damage. The extract exhibited metal binding ability and scavenging activity for hydrogen peroxide and hydroxyl radical, which may explain the mechanism of their protecting lipids and non-lipids from oxidative damage.