Effect of the natural antioxidant 2″-O-glycosylisovitexin on superoxide and hydroxyl radical generation

The inhibitory effect of 2″-O-glycosyl isovitexin (2″-O-GIV) isolated from green barley leaves on superoxide and hydroxyl radical generation was investigated using electron spin resonance spectroscopy. 2″-O-GIV exhibited dose-related inhibitory activity both towards superoxide generation and hydroxyl radical generation. 2″-O-GIV inhibited superoxide formation by 97% at a level of 25 mm and inhibited hydroxyl radical formation by 91% at a level of 500 μm. The results suggest that the inhibitory activity of 2″-O-GIV is due to trapping radicals.