Title of article :
Estrogenic and clastogenic potential of the mycotoxin alternariol in cultured mammalian cells
Author/Authors :
Lehmann، نويسنده , , L. and Wagner، نويسنده , , J. D. Metzler، نويسنده , , M.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2006
Pages :
11
From page :
398
To page :
408
Abstract :
The mycotoxin alternariol (AOH) is found in food and beverages infected by Alternaria alternata. Because consumption of foodstuffs contaminated with A. alternata has been implicated in an elevated incidence of esophageal carcinogenesis, we have investigated the estrogenic potential, the effect on cell proliferation, and the genotoxic effect of AOH in cultured mammalian cells. placed E2 from isolated human estrogen receptors α and β and increased the level of alkaline phosphatase (ALP) mRNA and the enzymatic activity of ALP in a human endometrial adenocarcinoma cell line (Ishikawa cells). The estrogenicity of AOH was about 0.01% of that of E2. The effects in Ishikawa cells were reversed by the ER antagonist ICI 182,780. is of cell proliferation by flow cytometry and microscopy of Ishikawa and Chinese hamster V79 cells revealed that AOH inhibited cell proliferation by interference with the cell cycle. notoxic potential was assessed by the micronucleus (MN) assay and immunochemical differentiation between MN containing whole chromosomes (kinetochore-positive) and DNA fragments (kinetochore-negative) in Ishikawa and V79 cells. AOH induced kinetochore-negative MN in both cell lines. s the first report on the estrogenic potential, inhibition of cell proliferation and clastogenicity of AOH in Ishikawa and V79 cells in vitro.
Keywords :
Alternariol , Micronuclei , V79 cells , Estrogen , Ishikawa cells
Journal title :
Food and Chemical Toxicology
Serial Year :
2006
Journal title :
Food and Chemical Toxicology
Record number :
2118550
Link To Document :
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