Author/Authors :
Yang، نويسنده , , Hsin-Ling and Chen، نويسنده , , Ssu-Ching and Chang، نويسنده , , Nai-Wen and Chang، نويسنده , , Jia-Ming and Lee، نويسنده , , Mei-Ling and Tsai، نويسنده , , Pei-Chuan and Fu، نويسنده , , Han-Hsuan and Kao، نويسنده , , Wei-Wan and Chiang، نويسنده , , Hsiao-Chi and Wang، نويسنده , , Hsuan-Hui and Hseu، نويسنده , , You-Cheng، نويسنده ,
Abstract :
Bidens pilosa (B. pilosa) is well known in Taiwan as a traditional Chinese medicine. The purpose of this study was to evaluate the ability of both the ethanol (EtOH) and ethylacetate/ethanol (EA/EtOH) extracts from the whole B. pilosa plant, to protect normal human erythrocytes against oxidative damage in vitro. It was determined that the oxidative hemolysis and lipid/protein peroxidation of erythrocytes induced by the aqueous peroxyl radical [2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)] were suppressed by both EtOH (50–150 μg/ml) and EA/EtOH (25–75 μg/ml) extracts of B. pilosa in concentration- and time-dependent manners. B. pilosa extracts also prevented the decline of superoxide dismutase (SOD) activity and the depletion of cytosolic glutathione (GSH) and ATP in erythrocytes. These results imply that B. pilosa may have protective antioxidant properties.
Keywords :
Superoxide Dismutase , glutathione , Hemolysis , antioxidants , Bidens pilosa
