Title of article :
Anti-inflammatory effect of anemarsaponin B isolated from the rhizomes of Anemarrhena asphodeloides in LPS-induced RAW 264.7 macrophages is mediated by negative regulation of the nuclear factor-κB and p38 pathways
Author/Authors :
Kim، نويسنده , , Ji-Yeon and Shin، نويسنده , , Ji-Sun and Ryu، نويسنده , , Jong Hoon and Kim، نويسنده , , Sun Yeou and Cho، نويسنده , , Young-Wuk and Choi، نويسنده , , Jung-Hye and Lee، نويسنده , , Kyung-Tae، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2009
Pages :
8
From page :
1610
To page :
1617
Abstract :
Anemarrhena asphodeloides is widely used in traditional Chinese medicine, and is known to have anti-diabetic and diuretic effects. In this study, we evaluated the anti-inflammatory effects of anemarsaponin B (ASB), a steroidal saponin isolated from the rhizomes of A. asphodeloides (Liliaceae), in LPS-stimulated RAW 264.7 macrophage cell line. ASB significantly and dose-dependently decreased the protein and mRNA levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). ASB also reduced the expressions and productions of pro-inflammatory cytokines, including those of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Electrophoretic mobility shift assay (EMSA) and reporter gene assays revealed that ASB attenuated the LPS-induced DNA binding and transcriptional activity of nuclear factor-kappa B (NF-κB). In addition, it was found that pretreatment with ASB significantly inhibited the nuclear translocation of the p65 subunit of NF-κB by blocking the phosphorylation of inhibitory kappa B-α (IκB-α). On the other hand, ASB inhibited the phosphorylation of MAP kinase kinases 3/6 (MKK3/6) and mixed lineage kinase 3 (MLK3), which are both involved in the p38 pathway. Taken together, these results suggest that anti-inflammatory effect of ASB in LPS-treated RAW 264.7 macrophages is associated with the inhibition of NF-κB transcriptional activity, possibly via the p38 MAP kinase pathway.
Keywords :
Anemarsaponin B , inducible nitric oxide synthase , Cyclooxygenase-2 , Nuclear factor-?B , p38 MAP kinase
Journal title :
Food and Chemical Toxicology
Serial Year :
2009
Journal title :
Food and Chemical Toxicology
Record number :
2121024
Link To Document :
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