Toxic effect of melanoidins from glucose–asparagine on trypsin activity

In this work the effect of the presence of the melanoidins from glucose–asparagine on the enzymatic activity of trypsin is studied. It was observed that an excess of Nα-benzoyl-l-arginine ethyl ester (BAEE) has an inhibiting effect on this enzyme activity. The maximum reaction rate was obtained for a 0.06 mN substrate concentration. It is also observed that the presence of melanoidin inhibits the enzymatic activity of trypsin. This inhibition can be described as a linear mixed type where the inhibition constant αKi of the substrate–inhibitor complex is higher than the inhibition constant Ki of the complex enzyme–inhibitor with a α value of 1.88.