Title of article :
Mutagenic and genotoxic effects of carbosulfan in freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis
Author/Authors :
Nwani، C. D. نويسنده , , C.D. and Lakra، نويسنده , , W.S. and Nagpure، نويسنده , , N.S. Krishna Kumar، نويسنده , , Ravindra and Kushwaha، نويسنده , , B. and Srivastava، نويسنده , , S.K.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2010
Pages :
7
From page :
202
To page :
208
Abstract :
Carbosulfan insecticide is widely used in agriculture and was recently proposed for treatment against pyrethroid-resistant mosquitoes. The mutagenic and genotoxic effect of carbosulfan was carried out in fish Channa punctatus using micronucleus (MN) test and comet assay. The 96 h LC50, estimated by probit analysis in a semi-static bioassay experiment, was 0.268 mg l−1. Based on the LC50 value, three sub-lethal concentrations of carbosulfan (1/4th LC50 = ∼67 μg l−1, 1/2nd LC50 = ∼134 μg l−1 and 3/4th LC50 = ∼201 μg l−1) were selected and fishes were exposed to the said concentrations for 96 h and the samplings were done at regular intervals of 24 h for assessment of the MN frequencies and DNA damage. In general, significant effects (P < 0.01) from both concentrations and time of exposure were observed in exposed fishes. The MN induction was highest on 96 h at all the concentrations in the peripheral blood. Similar trend was observed for the DNA damage measured in terms of the percentage of tail DNA in the erythrocyte and gill cells. This study confirmed that the comet and micronucleus assays are useful tools in determining potential genotoxicity of water pollutants and might be appropriate as a part of monitoring program.
Keywords :
Carbosulfan , Channa punctatus , DNA damage , Comet assay , Micronucleus , Genotoxicity
Journal title :
Food and Chemical Toxicology
Serial Year :
2010
Journal title :
Food and Chemical Toxicology
Record number :
2121523
Link To Document :
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