Author/Authors :
Kim، نويسنده , , Ok Tae and Lee، نويسنده , , Jei Wan and Bang، نويسنده , , Kyong Hwan and Chang Kim، نويسنده , , Young and Yun Hyun، نويسنده , , Dong and Cha، نويسنده , , Seon Woo and Choi، نويسنده , , Yong Eui and Lan Jin، نويسنده , , Mei and Hwang، نويسنده , , Baik، نويسنده ,
Abstract :
To elucidate the exact function of CabAS in Centella asiatica, which was previously reported as a putative β-amyrin synthase [Plant Cell Rep, 24:304–311, 2005], this gene was functionally expressed in the lanosterol synthase-deficient yeast mutant (erg7). After inducing the CabAS gene with galactose, a peak consistent with the dammarenediol standard was detected in LC/APCIMS analyses and the accumulated product was confirmed as dammarenediol. CabAS should therefore be renamed to C. asiatica dammarenediol synthase (CaDDS). The confirmation of this gene function may allow us to better understand the generation of numerous triterpene carbon skeletons.
