Title of article :
Measurement of glycidol hemoglobin adducts in humans who ingest edible oil containing small amounts of glycidol fatty acid esters
Author/Authors :
Honda، نويسنده , , Hiroshi and Onishi، نويسنده , , Masayuki and Fujii، نويسنده , , Kenkichi and Ikeda، نويسنده , , Naohiro and Yamaguchi، نويسنده , , Tohru and Fujimori، نويسنده , , Taketoshi and Nishiyama، نويسنده , , Naohiro and Kasamatsu، نويسنده , , Toshio، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
Abstract :
Hemoglobin (Hb) adducts are frequently used to address and/or monitor exposure to reactive chemicals. Glycidol (G), a known animal carcinogen, has been reported to form Hb adducts. Here, we measure G adduct levels in humans who daily ingest DAG oil, an edible oil consisting mainly of diacylglycerol. Since DAG oil contains a small amount of glycidol fatty acid esters (GEs), possible exposure to G released from GEs has been raised as a possible concern. For measurement of Hb adducts, we employed the N-alkyl Edman method reported by Landin et al. (1996) using gas chromatography–tandem mass spectrometry with minor modifications to detect G-Hb adducts as N-(2,3-dihydroxy-propyl)valine (diHOPrVal). Blood samples were collected from 7 DAG oil users and 6 non-users, and then G-Hb adduct levels were measured. G-Hb adducts were detected in all samples. The average level of diHOPrVal was 3.5 ± 1.9 pmol/g globin in the DAG oil users and 7.1 ± 3.1 pmol/g globin in the non-users. We conclude that there is no increased exposure to G in individuals who daily ingest DAG oil.
Keywords :
DAG oil , Glycidol fatty acid ester , Hemoglobin adducts , Edible oil , Glycidol
Journal title :
Food and Chemical Toxicology
Journal title :
Food and Chemical Toxicology