Author/Authors :
-، - نويسنده Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University
P.O. Box 14115-143, Tehran, I.R. IRAN Vasheghani Farahani, Ebrahim , -، - نويسنده Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University
P.O. Box 14115-143, Tehran, I.R. IRAN Khosravi Darani, Kianoush , -، - نويسنده Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University
P.O. Box 14115-143, Tehran, I.R. IRAN Shojaosadati, Saied Abbas
Abstract :
The Taguchi design of experiments was used to test the relative importance of medium components and environmental factors on poly(β-hydroxybutyrate)(PHB) production by Ralstonia eutropha. The optimum condition was obtained as: fructose concentration, 15 g/L; C/N ratio, 7.4; agitation speed 200 rpm; culture time, 40 h; temperature, 25 ° C; seed age, 15 h. At optimum condition the yield of PHB production was found to be 92.36%.
