Effect of fluoridated bleaching gels on the remineralization of predemineralized bovine enamel in vitro

Objectives tudy evaluated possible differences regarding the remineralization of predemineralized enamel after exposure to fluoridated or non-fluoridated bleaching gels. s amel specimens were prepared from sixty bovine incisors; before and after demineralization (37 °C; pH 4.95; 10 d), one-quarter of each specimenʹs surface was covered with nail varnish (control sound/demineralized). Subsequently, the specimens were stored for 16 h daily in a remineralizing solution (pH 7.0; 14 d), while for the remaining time (8 h) various bleaching gels were applied: (1) no treatment (control), (2) Opalescence regular (O, Ultradent), (3) Opalescence PF (O-PF), (4) Nite White ACP (NW-ACP, Discus Dental) and (5) Nite White ACPF (NW-ACPF). Following, half of the bleached parts were nail-varnished and stored for another 3-weeks period in a remineralizing solution. Differences in mineral losses (ΔΔZ) and lesion depths (ΔLD) before and after treatment/remineralization period were evaluated from microradiographs. ΔΔZsurface values (mineral loss of the outer 18 μm of the lesion) were calculated. s 2 and 5 weeks ΔΔZ/ΔLD values of the bleaching groups did not differ significantly from the controls. Treatment with NW-ACPF for 2 weeks resulted in significantly lower ΔΔZ values compared to NW-ACP (p = 0.032) and NW-ACPF in higher values than Opalescence regular (p = 0.006). Two weeks treatment with O and O-PF resulted in decreased ΔΔZsurface values compared to control (p < 0.0005), whereas with NW-ACPF no significant differences could be observed (p = 0.062). Application of NW-ACP induced significantly increased ΔΔZsurface values compared to control (p = 0.001). sions porting influence of fluoride-containing bleaching gels on remineralization could be observed.